Reverse Phase Protein Arrays for Compound Profiling
نویسندگان
چکیده
منابع مشابه
Protein profiling in cancer cell lines and tumor tissue using reverse phase protein arrays
Reverse phase protein array (RPPA) technology is an antibody-based high-throughput assay for protein profiling of biological specimens that allows for many measurements with very small amounts of cell lysate. Here, we report the sensitivity, reproducibility, and accuracy of a particular RPPA platform called Zeptosens. We customized the RPPA protocol for our in-house setup, and measured more t...
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Reverse phase protein arrays represent a new proteomics microarray technology with which to study the fluctuating state of the proteome in minute quantities of cells. The activation status of cell signaling pathways controls cellular fate and deregulation of these pathways underpins carcinogenesis. Changes in pathway activation that occur between early stage prostatic epithelial lesions, prosta...
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Recent advances in high throughput, high content "omic" technologies coupled with clinical information has lead to the expectation that the complexity of the molecular information generated will lead to more robust scientific research as well as the expectation that overarching therapeutic approaches will be patient-tailored to the underlying specific molecular defects of the disease. As diseas...
متن کاملVariable slope normalization of reverse phase protein arrays
MOTIVATION Reverse phase protein arrays (RPPA) measure the relative expression levels of a protein in many samples simultaneously. A set of identically spotted arrays can be used to measure the levels of more than one protein. Protein expression within each sample on an array is estimated by borrowing strength across all the samples, but using only within array information. When comparing acros...
متن کاملUtility of reverse phase protein arrays: applications to signalling pathways and human body arrays.
Protein microarrays offer a new means by which to conduct quantitative profiling of disease-associated proteins. The knowledge gained may provide novel strategies for early detection, diagnosis and therapeutic intervention. A variety of sophisticated approaches, including gene arrays, sequencing consortiums and large-scale two-dimensional gel electrophoresis, continue to generate lists of prote...
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ژورنال
عنوان ژورنال: Current Protocols in Chemical Biology
سال: 2016
ISSN: 2160-4762,2160-4762
DOI: 10.1002/cpch.9